2/12/2024 0 Comments Vchat stainRed-stained inflammatory cells distribute to the edematous dermis (arrowheads). (D): Allergic dermatitis induced by the application of DNFB to the ears of mice. Lipid droplets in swollen hepatocytes (hc) show a considerable range of sizes, and very small lipid droplets are identifiable. (C): Hepatic steatosis induced using a methionine and choline-deficient diet. Note that stromal cells (*) contain very small blue-gray lipid droplets. Proliferating tumor cells (tc) with large oval cell bodies isolated from the tumor mass are also seen. Deposition of fine nascent collagen fibers is seen in the stroma (arrowheads). In the vessel, an endothelial cell inwardly projects a slender cytoplasmic process (arrow) to the opposite luminal surface, presumably to increase the number of vessels by splitting the lumen in the angiogenic process. A newly formed vessel (nv) can be detected. Nascent fine collagen fibers (arrowheads) are identifiable by pink staining. (A): Granulating tissue formed 5 days after the skin punching injury. Bar: 20 μm.Īppearance of pathological tissues. Many cartilage cells (cc) have gray-blue lipid droplets (*). The cytoplasm of cartilage cells stains medium purple. Cartilage matrix (cmx) stains deep purple. Note that the lipid droplets in hepatocytes tended to be small in size (~1 μm in diameter), whereas medium-sized droplets (~2–4 μm in diameter) were found in hepatic stellate cells (Ito cells) located in the space of Disse. Around a sinusoid, thin reticular fibers stained pink can be observed. Red-stained elastic fibers with a wavy pattern are highlighted (arrowheads). In the tunica media, red-stained elastic fibers (arrowheads) are recognizable in between smooth muscles (*), while collagen fibers (cf) in the tunica adventitia stain pink. Fibroblasts accumulate at the junction (arrowheads) presumably to connect the muscles and the tendon strongly by collagen secretion. Skeletal muscle cells (mc) stain purple, while collagen fibers (cf) appear pink. (A): Junction between the soleus muscle and the calcaneal tendon. Bar: 10 μm.Īppearance of extracellular elements. Mucus-containing secretory granules (*) appear faint pink. (H): Cytoplasm of a goblet cell (g) in the jejunum is stained deeper reddish purple than neighboring absorptive epithelial cells. In the region next to the pore of the hair follicle (hf), sebum vesicles merge and form a lump of sebum (*). Sebaceous glandular cells (sg) contain numerous lipid-rich sebum vesicles that are stained gray-blue. The granules of a mast cell (arrowhead) are stained ultramarine blue or nearly black. A tiny conjunctive lipid droplet (arrow) formed next to a large droplet. Adipose cells contain gray-blue-stained large lipid droplets (lp). Numerous mitochondria of various cross-sectioned shapes stain deep purple and are tightly packed in myocardial cells (mc). A neutrophil (arrow) has a medium purple cytoplasm. A lymphocyte (arrow) is migrating through the endothelium. Neutrophils (arrowheads) have red-stained cytoplasm. A lymphocyte is captured within the cell (arrow). Lysosomes of various sizes stain red (arrowheads). Nuclear chromatin (*) stains denser than the cytoplasm. Leydig cells (lc) contain lipid droplets that stain gray-blue. This technique represents a simple polychrome-staining method to allow more informative and convincing histological investigation in various fields of research and education.Īppearance of cellular elements. Our method enabled clear differentiation of various tissue structures according to color tone and stain intensity, thereby facilitating the detection of fine structural differences, including various organelle and inclusion bodies. We optimized various staining conditions to enable sufficient coloration easily and consistently in a single, rapid staining step, using a single staining-mixture solution. We stained preheated sections with an aqueous ethanol solution of azure B and basic fuchsin, with the addition of sodium tetraborate to enhance the staining efficacy. In this study, we developed a simple polychromatic staining method for epoxy-embedded tissue sections. Additionally, polychromatic staining methods generally require step-by-step processes involving different dyes, and it is often difficult to balance the color tone of each step. Although conventional toluidine blue staining is a common technique used for rapid observation of semithin sections prior to transmission electron microscopy, it is monochromatic and insufficient for accurate identification of different tissue components by light microscopy.
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